8 research outputs found

    Adaptive multimedia streaming control algorithm in wireless LANs and 4G networks

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    E-learning has become an important service offered over the Internet. Lately many users are accessing learning content via wireless networks and using mobile devices. Most content is rich media-based and often puts significant pressure on the existing wireless networks in order to support high quality of delivery. In this context, offering a solution for improving user quality of experience when multimedia content is delivered over wireless networks is already a challenging task. Additionally, to support this for mobile e-learning over wireless LANs becomes even more difficult. If we want to increase the end-used perceived quality, we have to take into account the users’ individual set of characteristics. The fact that users have subjective opinions on the quality of a multimedia application can be used to increase their QoE by setting a minimum quality threshold below which the connection is considered to be undesired. Like this, the use of precious radio resources can be optimized in order to simultaneously satisfy an increased number of users. In this thesis a new user-oriented adaptive algorithm based on QOAS was designed and developed in order to address the user satisfaction problem. Simulations have been carried out with different adaptation schemes to compare the performances and benefits of the DQOAS mechanism. The simulation results are showing that using a dynamic stream granularity with a minimum threshold for the transmission rate, improves the overall quality of the multimedia delivery process, increasing the total number of satisfied users and the link utilization The good results obtained by the algorithm in IEEE 802.11 wireless environment, motivated the research about the utility of the newly proposed algorithm in another wireless environment, LTE. The study shows that DQOAS algorithm can obtain good results in terms of application perceived quality, when the considered application generates multiple streams. These results can be improved by using a new QoS parameters mapping scheme able to modify the streams’ priority and thus allowing the algorithms decisions to not be overridden by the systems’ scheduler

    Spatio-temporal insights into microbiology of the freshwater-to-hypersaline, oxic-hypoxic-euxinic waters of Ursu Lake

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    Ursu Lake is located in the Middle Miocene salt deposit of Central Romania. It is stratified, and the water column has three distinct water masses: an upper freshwater-to-moderately saline stratum (0–3 m), an intermediate stratum exhibiting a steep halocline (3–3.5 m), and a lower hypersaline stratum (4 m and below) that is euxinic (i.e. anoxic and sulphidic). Recent studies have characterized the lake's microbial taxonomy and given rise to intriguing ecological questions. Here, we explore whether the communities are dynamic or stable in relation to taxonomic composition, geochemistry, biophysics, and ecophysiological functions during the annual cycle. We found: (i) seasonally fluctuating, light-dependent communities in the upper layer (≥0.987–0.990 water-activity), a stable but phylogenetically diverse population of heterotrophs in the hypersaline stratum (water activities down to 0.762) and a persistent plate of green sulphur bacteria that connects these two (0.958–0.956 water activity) at 3–3.5 to 4 m; (ii) communities that might be involved in carbon- and sulphur-cycling between and within the lake's three main water masses; (iii) uncultured lineages including Acetothermia (OP1), Cloacimonetes (WWE1), Marinimicrobia (SAR406), Omnitrophicaeota (OP3), Parcubacteria (OD1) and other Candidate Phyla Radiation bacteria, and SR1 in the hypersaline stratum (likely involved in the anaerobic steps of carbon- and sulphur-cycling); and (iv) that species richness and habitat stability are associated with high redox-potentials. Ursu Lake has a unique and complex ecology, at the same time exhibiting dynamic fluctuations and stability, and can be used as a modern analogue for ancient euxinic water bodies and comparator system for other stratified hypersaline systems

    Limnology and plankton diversity of salt lakes from Transylvanian Basin (Romania): A review

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    In the present work, we review the current knowledge on genesis, limnology and biodiversity of salt lakes distributed around the inner contour of Eastern Carpathian arc (Transylvanian Basin, Central Romania). Transylvanian salt lakes formed on ancient halite (NaCl) deposits following natural processes or quarrying activities.  Most of these lakes are located in eastern (Sovata area), southern (Ocna Sibiului), and western (Turda-Cojocna) parts of the Transylvanian Basin, have small surfaces (0.1-4 ha), variable depths (2-100 m), are hypersaline (>10%, w/v, total salts, mainly NaCl) and permanently stratified. As consequence of steady salinity/density gradient, heat entrapment below surface layer (i.e., heliothermy) develops in several Transylvanian lakes. The physical and chemical water stratification is mirrored in the partition of plankton diversity. Lakes with less saline (2-10% salinity) water layers appear to harbor halotolerant representatives of phyto- (e.g., marine native Picochlorum spp. and Synechococcus spp.), zoo- (e.g., Moina salina), and bacterioplankton (e.g., Actinobacteria, Verrucomicobia), whereas halophilic plankton communities (e.g., green algae Dunaliella sp., brine shrimp Artemia sp., and members of Halobacteria class) dominate in the oxic surface of hypersaline (>10% salinity) lakes. Molecular approaches (e.g., PCR-DGGE, 16S rRNA gene-based clone libraries, and DNA metabarcoding) showed that the O2-depleted bottom brines of deep meromictic Transylvanian lakes are inhabited by known extremely halophilic anaerobes (e.g. sulfate-reducing Delta-Proteobacteria, fermenting Clostridia, methanogenic and polymer-degrading archaea) in addition to representatives of uncultured/unclassified prokaryotic lineages. Overall, the plankton communities thriving in saline Transylvanian lakes seem to drive full biogeochemical cycling of main elements. However, the trophic interactions (i.e., food web structure and energy flow) as well as impact of human activities and predicted climate changes are yet to be assessed in these unique ecosystems with little or no match to analogous salt lakes worldwide

    Data from: Contrasting taxonomic stratification of microbial communities in two hypersaline meromictic lakes

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    Hypersaline meromictic lakes are extreme environments in which water stratification is associated with powerful physicochemical gradients and high salt concentrations. Furthermore, their physical stability coupled with vertical water column partitioning makes them important research model systems in microbial niche differentiation and biogeochemical cycling. Here, we compare the prokaryotic assemblages from Ursu and Fara Fund hypersaline meromictic lakes (Transylvanian Basin, Romania) in relation to their limnological factors and infer their role in elemental cycling by matching taxa to known taxon-specific biogeochemical functions. To assess the composition and structure of prokaryotic communities and the environmental factors that structure them, deep-coverage small subunit (SSU) ribosomal RNA (rDNA) amplicon sequencing, community domain-specific quantitative PCR and physicochemical analyses were performed on samples collected along depth profiles. The analyses showed that the lakes harbored multiple and diverse prokaryotic communities whose distribution mirrored the water stratification patterns. Ursu Lake was found to be dominated by Bacteria and to have a greater prokaryotic diversity than Fara Fund Lake that harbored an increased cell density and was populated mostly by Archaea within oxic strata. In spite of their contrasting diversity, the microbial populations indigenous to each lake pointed to similar physiological functions within carbon degradation and sulfate reduction. Furthermore, the taxonomy results coupled with methane detection and its stable C isotope composition indicated the presence of a yet-undescribed methanogenic group in the lakes’ hypersaline monimolimnion. In addition, ultrasmall uncultivated archaeal lineages were detected in the chemocline of Fara Fund Lake, where the recently proposed Nanohaloarchaeota phylum was found to thrive

    Spatial Distribution and Molecular Diversity of Archaeal Communities in the Extreme Hypersaline Meromictic Brâncoveanu Lake (Transylvanian Basin, Romania)

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    <p>Dating from the Middle Miocene, the massive halite deposits lying beneath the Transylvanian Basin (Central Romania) have been valuable mineral resources quarried for millennia. Among the numerous hypersaline pit lakes that resulted from this mining, Brâncoveanu Lake is unique by its extreme salinity. Assessment of physicochemical variables, water chemistry and trophic status indicated that Brâncoveanu Lake is a permanently stratified, pH-neutral, NaCl-rich and eutrophied system. We investigated the abundance, molecular diversity and vertical distribution of archaeal community by culture-independent approaches. Additionally, the most relevant environmental parameters shaping the archaeal community composition were evaluated by statistical methods. Archaea appeared to largely outnumber Bacteria; altogether the great prevalence of Halobacteriaceae-related sequences could imply a major contribution of this group to the biogeochemical carbon turnover. The fairly distinct composition of archaeal communities reflects the lake's physicochemical stratification. Among the limnological factors, salinity and oxygen showed a significant impact on determining the composition and structure of archaeal assemblages. Furthermore, Brâncoveanu Lake might harbor novel microorganisms such as members of the recently described phylum Nanohaloarchaea. Overall, this study reported the occurrence of halophilic Archaea in a little explored hydrogeochemical system and provided a better insight into geomicrobiology of meromictic hypersaline pit lakes.</p

    Spatio-temporal insights into microbiology of the freshwater-to-hypersaline, oxic-hypoxic-euxinic waters of Ursu Lake

    No full text
    Ursu Lake is located in the Middle Miocene salt deposit of Central Romania. It is stratified, and the water column has three distinct water masses: an upper freshwater-to-moderately saline stratum (0–3 m), an intermediate stratum exhibiting a steep halocline (3–3.5 m), and a lower hypersaline stratum (4 m and below) that is euxinic (i.e. anoxic and sulphidic). Recent studies have characterized the lake's microbial taxonomy and given rise to intriguing ecological questions. Here, we explore whether the communities are dynamic or stable in relation to taxonomic composition, geochemistry, biophysics, and ecophysiological functions during the annual cycle. We found: (i) seasonally fluctuating, light-dependent communities in the upper layer (≥0.987–0.990 water-activity), a stable but phylogenetically diverse population of heterotrophs in the hypersaline stratum (water activities down to 0.762) and a persistent plate of green sulphur bacteria that connects these two (0.958–0.956 water activity) at 3–3.5 to 4 m; (ii) communities that might be involved in carbon- and sulphur-cycling between and within the lake's three main water masses; (iii) uncultured lineages including Acetothermia (OP1), Cloacimonetes (WWE1), Marinimicrobia (SAR406), Omnitrophicaeota (OP3), Parcubacteria (OD1) and other Candidate Phyla Radiation bacteria, and SR1 in the hypersaline stratum (likely involved in the anaerobic steps of carbon- and sulphur-cycling); and (iv) that species richness and habitat stability are associated with high redox-potentials. Ursu Lake has a unique and complex ecology, at the same time exhibiting dynamic fluctuations and stability, and can be used as a modern analogue for ancient euxinic water bodies and comparator system for other stratified hypersaline systems
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